All flies that emerge after the parents are removed are F1 generation and are placed into a new vial with fresh medium. Equal volume of water was then added to the dry mix. These larvae will then pupae for about 4 days before emerging adults. The number of wild type and ebony phenotype present within gender groups was then recorded.
H Morgan saw what Castle was doing with the fruit flies and began to use them as well. Females were placed into a newly prepared vial with medium, making sure not Genetics lab report drosophila mix wild type and ebony, and males into another.
Drosophila is widely used for education because it is a fantastic model organism. Geiger, There are many mutant variations of Drosophila with mutations in eye pigment, body pigment, body bristles and wing shape or size. Once the fly is asleep, we observed it under a dissecting scope or magnifying glass to determine gender.
We then took the swab and pressed the non-cotton end into the cotton stopper and closed the vial with the swab inside.
From this he was able to conclude that the inheritance of each trait is determined by units genesthat individuals inherit one unit from each parent for each trait, and that while traits may not show up in the individual they can still be passed to the next generation. This way there are enough fumes in the vial to knock out the fly but there is less risk of killing it.
By breeding this mutant with the normal red eye flies Morgan showed that the white eyes were a sex-linked mutation and went on to explain sex-linked inheritance and chromosome theory. To obtain females the vials need to be monitored closely and as soon as a new fly emerges it has to be isolated and sexed.
The same was done for ebony females with wild type males. Gregor Mendel, an Austrian monk, observed heritable traits in the common pea plant and discovered several key basic principles in Genetics.
This is done by taking plastic vials 1. Our group was given the mutant type Ebony which has a black body.
What he discovered was that there are 7 inheritable traits that only have 2 variations.
Once sleep, we separated them under a dissecting scope or magnifying glass by gender. While Mendel was the first to somewhat correctly explain genetic inheritance inhis work went unrecognized for nearly 40 years.
After 7 to 10 days, once larvae are present, the parents from the vials are removed. To do so we first anesthetize them. Once a number of vials were set up and breeding well, we began to separate out virgin females from both the wild type and the ebony Drosophila.
After about 20 seconds we removed the stopper and replaced it will a clean one. Once the F2 generation starts to emerge they are counted. First thing we did was set up additional stocks of both types of drosophila.
This is the P generation. Drosophila can go from egg to adult in just 12 days with a few other stages in between. Once we have about 6 virgin wild type females in the vial, we isolated 6 ebony males and placed them in to the vial.ABSTRACT As an introduction to Medallion genetics, the purpose of this lab is to discover how certain traits are inherited in the Drosophila melanogaster fly, and to understand the connection between presence or absence of a genetic trait in an individual and the ratio of those phenotypic traits in two generations%(50).
The F1 data that was collected for cross set C is indicated in Table 1. The number of flies for each phenotype is shown. Figure 1: Graphical representation of F1 data for cross set C.
Figure 1 presents the data from Table 1 in the form of a bar graph. Genetics of. Drosophila Lab Report Victor Martin April 14, Fourth Block AP Biology INTRODUCTION Genetics is a topic that has been studied for hundreds of years.5/5(3). A Model Organism: Drosophila melanogaster is used in this lab as well as many other wet-lab experiments, particularly genetic experiments, because it meets all the criteria in order to be a model organism.
LAB 11 – Drosophila Genetics Introduction: Drosophila melanogaster, the fruit fly, is an excellent organism for genetics studies because it The Results section of your lab report will include: A written summary of all data.
For each cross, you should mention total numbers Lab 11 Drosophila melanogaster. Introduction: The Drosophila melanogaster, the common fruit fly, has been used in genetics since for primary research (Genetics Laboratory Manual).
The first person to use the D. melanogaster was Thomas Hunt Morgan to show that Mendel's Law works in animals (Genetics Laboratory Manual).Download